Optimization of microclonal propagation conditions for increasing the multiplication factor of poplar microshoots

Abstract

Representatives of the genus poplar – Populus L. (kind Salicaceae) are widely used for landscape gardening residential areas and creating different types of protective plantings. Difficulties of propagation for some types of poplars by traditional methods is their weak rooting, as well as a high level of bacterial and fungal infection rate. Therefore using such methods as clonal micropropagation of plants in aseptic conditions on artificial nutrient medium is relevant for valuable forms of poplar. The purpose of this work is optimization of the conditions of microclonal propagation for increasing the coefficient of propagation of microshoots of Populus alba L. and Populus bolleana L. from axillary shoots introduced into an in vitro culture. The main objective set for solving this purpose were the regeneration of plants on the basis of direct proliferation of axillary meristem, their rooting and multiplication of the microshoots. High regeneration of axillary basic shoots of two types of poplars occurred on a WPM nutrient medium with the addition of the hormones BA (0.5 mg/l) and GA 0.2 mg/l. The WPM nutrient medium with the addition of BA hormones of 0.2 mg/l and 0.2 mg/l appropriate for increasing the quantity of shoots from axillary shoots. More optimal for rooting and growth of microshoots of Populus alba L. and Populus bolleana L. is Ѕ WPM nutrient medium for woody cultural with the addition of the hormone IBA 0.01 mg/l. Thereby, conditions of microclonal propagation was been optimized for increasing the coefficient of propagation of poplar microshoots.