Abstract:
Accurate and timely diagnosis of plant viral infections plays a key role in effective
disease control and maintaining agricultural productivity. Recent advances in the
diagnosis of plant viruses have significantly expanded our ability to detect and
monitor viral pathogens in agricultural crops. This review discusses the latest
advances in diagnostic technologies, including both traditional methods and the
latest innovations. Conventional methods such as enzyme-linked
immunosorbent assay and DNA amplification-based assays remain widely used
due to their reliability and accuracy. However, diagnostics such as nextgeneration sequencing and CRISPR-based detection offer faster, more
sensitive and specific virus detection. The review highlights the main
advantages and limitations of detection systems used in plant viral diagnostics
including conventional methods, biosensor technologies and advanced
sequence-based techniques. In addition, it also discusses the effectiveness of
commercially available diagnostic tools and challenges facing modern diagnostic
techniques as well as future directions for improving informed disease
management strategies. Understanding the main features of available
diagnostic methodologies would enable stakeholders to choose optimal
management strategies against viral threats and ensure global food security.
