Impact of combined abiotic and biotic stresses on ROS accumulation in Nicotiana benthamiana

Abstract

The effect of biotic and abiotic stress reduces and limits crop yields. In this study, molybdenum created an abiotic stress condition, while biotic stress was generated by inoculation of Tomato Bushy Stress Virus (TBSV) in Nicotiana benthamiana. TBSV is a model system in virological studies of the activity of plant viruses, especially in most cases the model host plant is Nicotiana benthamiana. Under various stress conditions, reactive oxygen species (ROS) components, especially hydrogen peroxide (H2O2), are accumulated, damaging cellular components (nucleic acids, lipids and proteins). In response to stress, plants have developed various mechanisms to cope with these threats. The purpose of this study is to demonstrate the effect of the combined effect of molybdenum and TBSV on N. benthamiana and its ROS accumulation.