Abstract:
Euonymus koopmannii is a rare and protected species in Kazakhstan, valued for its ecological
role in soil stabilization and its ornamental properties. This study presents the first use of micropropagation and phylogenetic analysis for the endemic plant E. koopmannii. Seedlings of E. koopmannii
proved to be more effective than internodes as primary explants for plant micropropagation of
in vitro culture, with a multiplication coefficient of 28.5 from seedlings and 6.1 from internodes. On
MSR I medium supplemented with 0.5 mg/L IBA and 0.05 mg/L IAA, a higher success rate of
67% was achieved for root formation of test tube-grown E. koopmannii plants. Using mannitol as an
osmotic agent at a concentration of 8 mg/L prolonged the storage time of E. koopmannii under slow
growth conditions when compared to CCC and abscisic acid. Phylogenetic relationships and species
identification were analyzed using four DNA-barcoding markers, comparing E. koopmannii with
species from NCBI. All candidate barcoding markers showed sufficient levels of interspecific genetic
variation among Euonymus species. In addition, ITS region and rbcL gene sequences effectively
distinguished E. koopmannii from other species. These results provide fundamental information that
will be valuable for future biotechnological and molecular studies.
