Abstract:
CRISPR-Cas and CRISPR-associated systems (CRISPR-Cas) are key immune mechanisms that allow prokaryotic species to ward off RNA and DNA viruses. CRISPR/Cas9 has broad biotechnology and basic research applications, as well as being widely used across eukaryotic species for genome engineering and functional gene analysis. Newly developed CRISPR/Cas13 systems target RNA rather than DNA and have the potential to be used for gene engineering and to combat RNA viruses. This paper provides an overview of the CRISPR/Cas system and discusses the guided RNA. We then describe different types of programs used to detect secondary structure of guided RNA in CRISPR/CAS13.
