Abstract:
Exposure to ionizing radiation can result in the development of a number of diseases,
including cancer, cataracts and neurodegenerative pathologies. Certain occupational groups are
exposed to both natural and artificial sources of radiation as a consequence of their professional
activities. The development of non-invasive biomarkers to assess the risk of exposure to ionizing
radiation for these groups is of great importance. In this context, our objective was to identify
epigenetic and molecular biomarkers that could be used to monitor exposure to ionizing radiation.
The impact of X-ray exposure on the miRNAs profile and the level of cf mtDNA were evaluated
using the RT-PCR method. The levels of pro-inflammatory cytokines in their blood were quantified
using the ELISA method. A significant decrease in miR-19a-3p, miR-125b-5p and significant increase
in miR-29a-3p was observed in the blood plasma of individuals exposed to X-ray. High levels of
pro-inflammatory cytokines and cf mtDNA were also detected. In silico identification of potential
targets of these miRNAs was conducted using MIENTURNET. VDAC1 and ALOX5 were identified
as possible targets. Our study identified promising biomarkers such as miRNAs and cf mtDNA that
showed a dose-dependent effect of X-ray exposure.
